Figure 2.

Verification of Tubulin Alpha and Neutrophil Defensin as LC-17-Associated Protein Targets

(I) Co-staining of the tissue sections with Alexa Fluor 405-labeled anti-tubulin alpha (A) and anti-neutrophil defensin (B) antibodies and Cy-5 labeled aptamer LC-17. (1) H&E staining of the adjacent section, (2) staining with correspondent Alexa Fluor 405-labeled antibody, (3) Cy-5-labeled aptamer LC-17, (4) overlay, and (5) histogram of fluorescence intensity are shown. Magnification, 40×. (II) Flow cytometry histograms indicating binding of aptamer LC-17 to lung adenocarcinoma cells and its replacement with anti-tubulin alpha (A) and anti-neutrophil defensin (B) antibodies. Red curve corresponds to intact lung adenocarcinoma cells, blue curve corresponds to cells bound with aptamer LC-17 (50 nM), and green curve corresponds to the same sample after incubation with 2 ng μL⁻¹ antibodies for 30 min with shaking at 37°C.