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. 2016 Dec 31;6:183–197. doi: 10.1016/j.omtn.2016.12.005

Figure 2.

Figure 2

TTF-1-Promoter-Operating miR-7 Expression Suppressed Tumorigenesis of Lung Cancer In Vivo

(A) The schedule of study design. (B) Human lung cancer cell line 95D cells were injected subcutaneously into right flank of BALB/c nude mice (n = 8). 7 days later, the plasmid of p-T-miR-7 (100 mg) or p-Cont (100 mg) was remotely given by subcutaneous injection into the left flank of nude mice five times every 3 days. 3 days after last injection, all mice were killed. The skin of injection sections was stained with H&E and observed by microscope (magnification 200×). And partial mice were imaged (C). (D) The growth curve of tumor. (E) The representative morphology and (F) weight of tumor. The expression of miR-7 in tumor tissue was detected by real-time PCR assay (G) and in situ hybridization assay (H). H&E staining of tumor tissue (I) and lung tissue (J). (K) The ability of proliferation (Ki-67) and apoptosis (TUNEL) in tumor tissue were analyzed by immunofluorescence assay. (L) The expression of cell-growth-related molecules (CDK2, CDK3, CDK4, and CDK6) and metastasis-related molecules (CXCR4, E-Cadherin, MMP2, MMP3, and MMP9) in tumor tissue was detected by real-time PCR assay. *p < 0.05, **p < 0.01. ***p < 0.001.