Figure 3.

TTF-1-Promoter-Operating miR-7 Expression Altered the Transduction of the Akt and Erk Signaling Pathway

Human lung cancer cell line 95D cells were injected subcutaneously into right flank of BALB/c nude mice (n = 8). 7 days later, the plasmid of p-T-miR-7 (100 mg) or p-Cont (100 mg) was remotely given by subcutaneous injection into the left flank of nude mice five times every 3 days. 3 days after last injection, tumor mass was collected. The protein level of Akt, p-Akt, Erk, and p-Erk was detected by western blotting (A) and calculated (B). Human lung cancer cell line 95D cells were transiently transfected with p-T-miR-7 (2 μg) or p-Cont (2 μg) in vitro. 48 hr later, cells were collected, and the protein level of Akt, p-Akt, Erk, and p-Erk was detected by western blotting (C) and calculated (D). *p < 0.05, **p < 0.01. Representative data of three independent experiments are shown.