Figure 5.

Overexpression of NDUFA4 Promoted the Proliferation and Migration of Human Lung Cancer Cells

(A) The sketch map of an eukaryotic expression vector encoding NDUFA4 (termed as p-NDUFA4). (B) Human lung cancer cell line 95D cells were transiently transfected with p-NDUFA4 (10 μg) or p-Cont (10 μg) in vitro. 48 hr later, the morphology of cells were observed by microscopy (magnification 100×). (C) The relative expression of NDUFA4 was determined by real-time PCR. (D) The proliferation of cells was detected by CCK-8 assay. (E) The migration of 95D cells was performed by scratch assay, and the cell number was also calculated. The yellow line indicates the border of scratch wound (magnification 100×). (F) The colon-formation assay also was performed and calculated at indicated time points. Human lung cancer cell line 95D cells were transiently transfected with p-NDUFA4 (10 μg) or p-Cont (10 μg) in vitro. 48 hr later, the expression of indicated cell-growth-related molecules and metastasis-related molecules was detected by real-time PCR assay (G). (H) The protein level of NDUFA4, Akt, p-Akt, Erk, and p-Erk was also detected by western blotting and calculated, respectively. Representative data of three independent experiments are shown.*p < 0.05, **p < 0.01.