Figure 6.

Overexpression of NDUFA4 Abrogated the Suppressive Effect of TTF-1-Promoter-Operating miR-7 Expression

Human lung cancer cell line 95D cells were transiently co-transfected with p-T-miR-7 (10 μg) and p-NDUFA4 (10 μg) in vitro. 48 hr later, (A) the morphology of cells were observed by microscopy (magnification 100×). (B) The proliferation of 95D cells was detected by CCK-8 assay. (C) The migration of 95D cells was performed by scratch assay, and the cell number was also calculated. The yellow line indicates the border of scratch wound (magnification 100×). (D) The colony-formation assay was also performed, and the colon numbers were calculated. (E) The relative expression of NDUFA4 was determined by real-time PCR. (F) The relative expression of indicated cell-growth-related molecules and metastasis-related molecules was also detected by real-time PCR assay. (G) The protein level of NDUFA4, Akt, p-Akt, Erk, and p-Erk was detected by western blotting and calculated, respectively. Representative data of three independent experiments are shown.*p < 0.05, **p < 0.01.