Figure 3.

Effect of WPRE in Expression Patterns of GFP in Retinal Layers following Subretinal Injection of AAV2-GFP ± WPRE in C57BL/6J Mice

(A–H) Confocal stacks were prepared from histological sections from eyes injected with AAV2-GFP ± WPRE at 2E+08 gc (A–D) and 1E+09 gc (E–H). GFP signal (green) and nuclear labeling with Hoechst (blue) were overlaid with the differential interference contrast (DIC) image to demonstrate the retinal layers. Scale bar, 25 μm. (I–M) Representative confocal slices were prepared from histological sections of an AAV2-GFP-WPRE-injected eye (1E+09 gc). GFP signal (K) and nuclear labeling (J) were overlaid with immunostaining using cone arrestin (L) and DIC (I) to demonstrate that GFP is expressed mainly in the RPE and rod outer segments. Scale bars, 25 μm. Detached areas are an artifact of the processing steps. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; ONL, outer nuclear layer; IS+OS, inner and outer segments of photoreceptors; RPE, retinal pigment epithelium.