Table 2.

Accumulation of Cy5.5-Labeled Ch-siRNA, Non-modified siRNA, and siRNA Complexed with Lipofectamine 2000 in the Organs of KB-8-5 Tumor-Bearing SCID Mice after i.v. Injection

	Total Fluorescence of the Organ (RFU)					Total Florescence of the Organ Relative to the Total Fluorescence of All Organs (%)
	Ch-siRNA			siRNA	siRNA+Lf	Ch-siRNA			siRNA	siRNA+Lf
	30 min	4 hr	24 hr	24 hr	24 hr	30 min	240 min	24 hr	24 hr	24 hr
Brain	7 ± 1	4.3 ± 1.6	0	0	0	2.7 ± 0.5	2.1 ± 0.7	0	0	0
Heart	3.9 ± 1.2	2.8 ± 0.6	0.6 ± 0.2	0	0	1.4 ± 0.4	1.3 ± 0.3	0.2 ± 0.1	0	0
Lungs	42 ± 8	23 ± 6	0.4 ± 0.3	0	0	15 ± 3	11 ± 3	0.1 ± 0.1	0	0
Liver	189 ± 27	154 ± 20	235 ± 54	2.1 ± 2.1	0.40 ± 0.02	70 ± 10	73 ± 9	76 ± 17	1.6 ± 1.6	6.7 ± 0.4
Kidneys	26 ± 4	25 ± 4	45 ± 6	122 ± 19	5.6 ± 1.3	9.7 ± 1.5	12 ± 2	15 ± 2	94 ± 14	93 ± 21
Spleen	0.5 ± 0.3	0.1 ± 0.1	8.2 ± 2.6	0.2 ± 0.3	0	0.2 ± 0.1	0.04 ± 0.05	2.7 ± 0.9	0.2 ± 0.2	0
Tumor	1.4 ± 0.5	2 ± 0.7	19 ± 4	5.4 ± 1.8	0	0.5 ± 0.2	0.9 ± 0.3	6.1 ± 1.3	4.1 ± 1.4	0
Total	270 ± 43	211 ± 32	308 ± 67	130 ± 23	6 ± 1.3	100	100	100	100	100

Mean ± SD (n = 3). Lf, Lipofectamine 2000.