Figure 2.

CRISPR-Cas9-Mediated Conversion of IDH2 R140Q Mutation from Primary AML Patient Blasts

(A) Schematic diagram illustrating lentiviral infection of human primary AML blasts with the lenti-CRISPR-Cas9 plasmid carrying sgRNA targeting the IDH2 gene as well as the IDLV plasmid carrying the DNA template for subsequent HR. (B) TI PCR confirming correct insertion of the DNA template at the IDH2 locus via HR. (C) Sanger DNA sequences of the genome modified AML patient samples after removal of the IDH2 R140Q mutation (right) and IDH2 wt sequence of the original patient sample as a control (left), respectively. Asterisks indicate the positions of the silent point mutations introduced to avoid CRISPR-Cas9-system-mediated re-cutting after successful genome editing.