Figure 4.

Signaling Pathway in Retinal NV Promoted by POSTN

HRECs were stimulated with POSTN and treated with or without control IgG, integrin αvβ3 Ab, αvβ5 Ab, or LY294002 (Akt inhibitor). (A) The phosphorylation of FAK-Tyr397, Akt-Ser473, ILK1 was increased at 15 min after POSTN stimulation. (B) Inhibition of integrin αvβ3 effectively reduced the FAK/Akt phosphorylation promoted by POSTN, whereas inhibition of integrin αvβ5 had no influence on the Akt phosphorylation. (C) The inhibition of integrin αvβ3 and Akt significantly suppressed the POSTN-induced migration (n = 12/group). *p < 0.0001. (D) Tube formation of HRECs was significantly inhibited by integrin αvβ3 Ab and Akt inhibitor (n = 4/group). *p < 0.0001, **p < 0.01, ***p < 0.05. Error bars are SEM.