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. 2016 Oct 11;7(47):76496–76507. doi: 10.18632/oncotarget.12562

Figure 2. Impact of AIF expression on the cytotoxicity of members of Quinone-derived drug family.

Figure 2

A.-C. U2OS cells transfected with two distinct control siRNAs (Co.1 and Co.2) or two distinct, non-overlapping siRNAs targeting AIF (siRNA AIF.1 and AIF.2) were treated with 120μM benzoquinone (BZQ) for 3h (A); 20 μM 2,3-dimethoxy-1,4-naphtoquinone (DMNQ) for 48h (B) or 6 μM mitoxantrone (MTX) for 19h (C) or the solvent (Control). Drug-induced cell death was quantified by flow cytometric assessment (histograms) of DAPI uptake (DAPI positivity) and forward light scatter (FSC) analysis that allows the identification of apoptotic cells according to their reduced size (low FSC). Data are expressed as mean values ± SEM.