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. 2016 Oct 25;7(47):76508–76522. doi: 10.18632/oncotarget.12894

Figure 5. DHM significantly increases FOXO3a expression, and enhances FOXO3a nuclear translocation and phosphorylation modification after I/R.

Figure 5

A. The mRNA level of FOXO3a. B. Representative immunoblot of FOXO3a protein levels, with β-actin as an internal loading control. C. Representative immunoblot of nuclear FoxO3a levels, with H3 as an internal loading control. D. p-FOXO3a(Ser588), p-FOXO3a(Ser7), p-FOXO3a(Ser294), p-FOXO3a(Ser253) and p-FOXO3a(Thr32) protein expression levels were measured using Western blot analysis as described previously, with β-actin as an internal loading control. E. Acetylation of FOXO3a by immunoprecipitation with an FOXO3a antibody, followed by immunoblot analysis of anti-acetylated-lysine. A representative Western blot and the quantification of the ratio of acetylated FOXO3a to FOXO3a are shown. The results are expressed as a percentage of the control, which was set at 100%. The values are presented as the means ± SEM, **p < 0.01 versus the control group and #p < 0.05, ##p < 0.01versus the liver I/R group (n = 20).