Table 1. Sequences of mouse primers used in quantitative RT-PCR.
Target gene | Primer | Nucleotide sequence |
---|---|---|
FOXO3a | F R |
5′-GGGGAACCTGTCCTATGCC-3′ 5′-TCATTCTGAACGCGCATGAAG-3′ |
BECN1 | F R |
5′-ATGGAGGGGTCTAAGGCGTC-3′ 5-TGGGCTGTGGTAAGTAATGGA-3′ |
ATG5 | F R |
5′-TGTGCTTCGAGATGTGTGGTT-3′ 5′-ACCAACGTCAAATAGCTGACTC-3′ |
ATG12 | F R |
5′-CGGAAGATTCAGAGGTTGTGCT-3′ 5′-CAGCCTTCAGCAGGATGTCAA-3′ |
LC3 | F R |
5′-TTATAGAGCGATACAAGGGGGAG-3′ 5′-CGCCGTCTGATTATCTTGATGAG-3′ |
β-actin | F R |
5′-CGTGCGTGACATCAAAGAGAAG-3′ 5′-CAAGAAGGAAGGCTGGAAAAGA-3′ |