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. 2016 Jul 6;7(47):76577–76589. doi: 10.18632/oncotarget.10428

Figure 2. PEM and Dasatinib affect cell viability, apoptosis, TS and SRC protein levels and cell migration.

Figure 2

PEM and dasatinib efficacy was established in all three MPM cell lines. REN and MPP89 (left and middle panels) were incubated with single and combination drugs for 72 hours, while 48 hours were sufficient for MSTO (right panel) (A). The apoptotic rate displayed differential responses, among MPM cells, to single and double treatments after 72 (left and middle graph) and 48 (right graph) hours, respectively (B). The same incubation times were applied to detect TS, SRC and pSRC protein expression (C). Wound healing assay was employed to determine the effect mediated by drugs on cell migration. Compared with untreated, dasatinib treatment abolished cell migration capability to heal the scrape after 24 hours in both REN and MPP89 cell lines (left and middle panels), and after 8 hours in MSTO (right panel). Plots display the progressive time-depending wound healing, in pixel units (px), under different drugs conditions (D).