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. 2016 Oct 1;7(47):76667–76683. doi: 10.18632/oncotarget.12395

Figure 11. miR-22 inhibits the translocation of NF-κB from the cytoplasm to the nucleus.

Figure 11

(A and B) U251 cells were transfected with miR-22 mimics (A), miR-22 inhibitors (B), or the corresponding control oligo (final concentration, 50 nM) for 24 h and then transfected with poly(I:C) for another 8 h. The cytosolic extracts (upper panel) and nuclear extracts (lower panel) were isolated and subjected to immunoblotting with antibodies against NF-κB (p65), LaminA, and GAPDH. LaminA was used as a marker for nuclei. GAPDH and LaminA were used as the loading control. Protein levels were quantified with immunoblot scanning and normalized to the amount of GAPDH or LaminA expressions. **p < 0.01; ***p < 0.001.