Skip to main content
. 2016 Oct 1;7(47):76667–76683. doi: 10.18632/oncotarget.12395

Figure 7. Overexpression of MAVS enhances the poly(I:C)-triggered production of IFN-β and inflammatory cytokines.

Figure 7

(A) U251 cells were co-transfected with miR-22 mimics or control mimics and pCDNA4 (200 ng) or pCDNA4-MAVS (200 ng) for 24 h and either left untransfected or transfected with 100 ng/ml poly(I:C). After 8 h, total RNA was extracted, and IFN-β level was determined with quantitative real-time PCR. (B–E) U251 cells were co-transfected with miR-22 mimics or control mimics and pCDNA4 (200 ng) or pCDNA4-MAVS (200ng) for 24 h and either left untransfected or transfected with 100ng/ml poly(I:C) for 8 h. The protein levels of TNF-α, IL-1β, and IL6 were analyzed by ELISA. Data represent means ± SD from three independent experiments performed in duplicate. (*p < 0.05; **p < 0.01). CCL5 mRNA levels were determined with quantitative real-time PCR and normalized to the expression of β-actin in each sample. All data are representative of at least three independent experiments. (*p < 0.05; **p < 0.01).