Table 2. Sequences of the primers used in the present study.
Primer | Sequence 5′→3′ |
---|---|
miR-22 stem-loop primer | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACAGTT |
miR-22-F | AAGCTGCCAGTTGAAGAACTGT |
Universal reverse primer | GTGCAGGGTCCGAGGT |
pri-miR-22-F | AGGAGTAGAAGGCTCAAACA |
pri-miR-22-R | AGGAGGGTCAAGAAGGAA |
pre-miR-22-F | GGCGAGCCGCAGAGC |
pre-miR-22-R | GGCGGGGCCGTTCTT |
hCCL5-F | CTGTCATCCTCATTGCTACTGC |
hCCL5-R | ATGTACTCCCGAACCCATTTCT |
hIFN-β-F | TTCCCAGGGCTTACACCG |
hIFN-β-R | TAACCCAAGTTCCCGAGT |
hMAVS-3′-UTR-XhoI-F | ACGCTCGAGAGCCCAGCCTGAGACCGT |
hMAVS-3′-UTR-PmeI-R | CGGTTTAAACCTGCACCCTTGACCACTGTG |
Mutant-primer-2-R | CTGACACGACCCCCTGGC |
Mutant-primer-3-F | AGCCAGGGGGTCGTGTC |
hMAVS-KpnI-F | CGAGGTACC ATGCCGTTTGCTGAAGACAAGACC |
hMAVS-XhoI-R | ACGCTCGAGGTGCAGACGCCGCCGGT |
h: human.