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. 2016 Oct 8;7(47):76756–76768. doi: 10.18632/oncotarget.12525

Figure 5. IL-6 elevation and STAT3 induction in cancer stem-like compartment of ESE3KD prostate epithelial cells.

Figure 5

(A) IL-6 mRNA level evaluated by qRT-PCR in adherent and prostatosphere cells derived from ESE3KD-PrECs (top) and ESE3KD-RWPE-1 (bottom) cells. Data are presented as fold change relative to the corresponding adherent cells. (B) ICC staining for STAT3 total and pSTAT3-Tyr705 for adherent cells and prostatospheres derived from ESE3KD-PrECs (upper) and ESE3KD-RWPE-1 (lower) cultures. (C) Flow cytometry analysis of STAT3-Tyr705 staining for indicated cells. Percentages of positive cells are shown.