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. 2016 Oct 8;7(47):76793–76805. doi: 10.18632/oncotarget.12529

Figure 5. Effects of tofacitinib on the expression of EBV-encoded genes in EBV-positive T and NK cell lines.

Figure 5

(A) EBV-positive T cell lines (SNT13, SNT15, and SNT16) and EBV-positive NK cell lines (KAI3 and SNK6) were treated with 5 μM tofacitinib and harvested at 0, 24 and 48 h to evaluate gene expression using real-time RT-PCR. BZLF1 is an immediate early gene and gp350/220 is a late gene in the lytic infection cycle of EBV. LMP1 and EBNA1 are latent EBV genes. β2-Microglobulin was used as an internal control and as a reference gene for relative quantification and was assigned an arbitrary value of 1 (10°). Values are geometric means ± SE of the results from six replicate experiments. *P < 0.05 as compared with DMSO-treated cells. (B) SNT13, SNT15, SNT16, KAI3 and SNK6 cell lines were treated with 5 μM tofacitinib for 48 h, and cell lysates were immunoblotted for LMP1, EBNA1 and BZLF1. Actin was blotted as a loading control.