Figure 4. Forced expression of CSMD1 decreases cell invasion and adhesive capacity.
(A–C) Cells capable of invading and migrating through a layer of matrigel to the underside of the cell culture insert membranes were photographed and counted after crystal violet staining for BT-20 (A), MDA-MB-231 (B) and T47D cells (C). Data are shown as the mean of cells counts ± SD from 3 independent experiments performed in single inserts. (D–F) Adherent cells to matrigel were photographed and counted after crystal violet staining for BT-20 (D), MDA-MB-231 (E) and T47D cells (F). Results shown are mean of cells counts ± SD from 3 independent experiments performed in at least four replicate. A one-way ANOVA was used to calculate statistical significance between the CTRL cells and CSMD1 expressing cells; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.