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. 2016 Oct 19;7(47):76995–77009. doi: 10.18632/oncotarget.12765

Figure 2. SPS-7 induces G1 arrest of the cell cycle and apoptosis.

Figure 2

(A) Synchronization of PC-3 cells was performed by thymidine block as described in the Materials and Methods section. Then, the cells were released in the absence or presence of 10 μM SPS-7 or FTY720 for the indicated times. Data are representative of three independent experiments. (B) Quantitative data are expressed as mean ± SEM of three independent experiments. (C) PC-3 or DU-145 cells were incubated in the absence or presence of the compound (10 μM) for 24 or 48 hours. After the treatment, the cells were harvested for the determination of sub-G1 population using the detection of DNA content analyzed with FACScan and CellQuest software. Data are expressed as mean ± SEM of three independent determinations. *P < 0.05, **P < 0.01 and ***P < 0.001 compared with the respective control.