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. 2016 Oct 19;7(47):76995–77009. doi: 10.18632/oncotarget.12765

Figure 6. FTY720 but not SPS-7 induces immunosuppressive effect.

Figure 6

CD2F1 mice were treated with or without intraperitoneal FTY720 or SPS-7 for 6 hours. Whole blood was collected and the spleens were dissected. (A) Splenic sections were fixed, embedded with paraffin and de-paraffinized for H&E staining. IHC staining was used for detecting S1PR expression and performed by using UltraVision™ Quanto Detection System HRP DAB (Thermo Fisher Scientific, Waltham, MA, USA) (A, figure inset). (B) Blood lymphocyte count was performed using specific CD marker antibody conjugation and removal of erythrocytesas described in the Materials and Methods section. Then, lymphocyte count was determined by flow cytometric analysis (Becton Dickinson, Mountain View, CA). Data are expressed as mean ± SEM of five independent experiments. *P < 0.05 compared with the control.