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. 2016 Oct 20;7(47):77038–77051. doi: 10.18632/oncotarget.12781

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Copyright: © 2016 Shimozaki et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fractions of phosphorylated GSK-3β (pGSK-3β^Y216: active form; pGSK-3β^S9: inactive form) and total GSK-3β were detected in protein extracts from osteosarcoma and hFOB1.19 cells by Western immunoblotting analysis. The amount of protein extract from each sample was compared to the expression of β-actin.