Skip to main content
. 2016 Oct 13;7(47):77257–77275. doi: 10.18632/oncotarget.12629

Figure 5. Target prediction of miR-503 and validation.

Figure 5

A. CXCL10/IP-10 was predicted to be one of the miR-503 targets according to TargetScan software, given the complementarity of CXCL10/IP-10 3′UTR and miR-503 seed sequence. B. 3′-UTR-Luc assay shows a significant decrease (about 50%, p value < 0.0001) in Luciferase reporter expression in transiently transfected 3′-UTR CXCL10/IP10 Luciferase stable 293 cell line with miR-503, as compared to the control vector, and a significant reversion is observed when a deleted form of the 3′-UTR CXCL10/IP-10 was used as specificity control. Data are reported as mean ± SD of 3 independent experiments.