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. 2016 Oct 13;7(47):77306–77318. doi: 10.18632/oncotarget.12639

Figure 2. DcR3 overexpression promoted CRC cell proliferation and migration in vitro.

Figure 2

A. RKO and HT29 cells were stably transfected with vector (Control) or DcR3 plasmids (DcR3). DcR3 plasmids effectively increased DcR3 expression in RKO and HT29 cells (Upper panel). DcR3 protein levels were normalized to α-Tubulin (Lower panel). ***P<0.001compared to control group, n=3. B-C. DcR3 overexpression promoted cell proliferation, as determined by the colony formation assay (B) and the CCK8 assay (C), *P<0.05 compared to control group at each corresponding time point; n=3. (D-E) DcR3 overexpression markedly increased RKO and HT29 cell migration, as determined by the transwell migration assay D. and the wound-healing assay E. ***P<0.001 compared to control group; n=3.