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. 2016 Oct 13;7(47):77306–77318. doi: 10.18632/oncotarget.12639

Figure 3. DcR3 promoted tumor growth and metastasis in vivo.

Figure 3

A-B. GFP-labeled RKO and HT29 cells stably transfected with vector (Ctrl) or DcR3 plasmids (DcR3) were injected subcutaneously into nude mice, as described in the Methods. Twenty-five days later, tumors were removed and imaged (Lower panels). Tumor growth curves were obtained using a whole-body GFP imaging system during tumor growth (Upper panels). Tumors derived from cells expressing DcR3 grew significantly larger than tumors derived from control cells. *P<0.001 compared to control group at each time point for both A and B; n=6. C-D. Representative photographs of H&E, DcR3 and Ki-67 immunohistochemistry staining of the primary tumor tissues from nude mice. E. GFP-labeled SW480/M5 cells stably transfected with control (shCtrl) or DcR3 shRNA (shDcR3) were injected into the tail veins of nude mice, as described in the Methods. Left: whole-lung images with metastatic foci, as indicated by the arrows. Right: H&E staining of lung sections showing metastatic tumors. F. Quantification of the numbers of metastatic foci in each mouse. More metastatic foci were detected in cells containing shCtrl than in cells containing shDcR3, ***P<0.001; n=6. G. DcR3 expression was positively correlated with survival time of nude mice, as shown by log-rank analysis; P=0.027.