Table 3. PCR primers and probe sequences.
Primers | Sequences |
---|---|
MiR-7 | |
Upstream | 5′-ACGCGGCAAGATGCTGGCA-3′ |
Downstream | 5′-CAGTGCTGGGTCCGAGTGA-3′ |
Probe | 5′-FAM-TCGTATCCAGTGCGAATGACTC-TAMRA-3′ |
FAK | |
Upstream | 5′-CAAGGCAGAAGGCAGCTATG-3′ |
Downstream | 5′-ATGGGAGGGAGCGTTTGA-3′ |
ERK | |
Upstream | 5′-AGTCGGAGTAGGGTGTTTCG-3′ |
Downstream | 5′-TTTTGCTTCTGGATGTGGG-3′ |
MAPK | |
Upstream | 5′-AAGTCACGCTGGAGTGGTTC-3′ |
Downstream | 5′-CCCCGTCATCAGTTCATACAA-3′ |
GAPDH | |
Upstream | 5′-TCCGGGTGATGCTTTTCCTAG-3′ |
Downstream | 5′-TTTGCGGTGGAAATGTCCTTTTC-3′ |
MiR-7, microRNA-7; FAK, focal adhesion kinase; ERK, extracellular signal-regulated kinase; MAPK, mitogen-activated protein kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PCR, polymerase chain reaction;