Skip to main content
. 2016 Oct 15;7(47):77468–77481. doi: 10.18632/oncotarget.12684

Table 3. PCR primers and probe sequences.

Primers Sequences
MiR-7
Upstream 5′-ACGCGGCAAGATGCTGGCA-3′
Downstream 5′-CAGTGCTGGGTCCGAGTGA-3′
Probe 5′-FAM-TCGTATCCAGTGCGAATGACTC-TAMRA-3′
FAK
Upstream 5′-CAAGGCAGAAGGCAGCTATG-3′
Downstream 5′-ATGGGAGGGAGCGTTTGA-3′
ERK
Upstream 5′-AGTCGGAGTAGGGTGTTTCG-3′
Downstream 5′-TTTTGCTTCTGGATGTGGG-3′
MAPK
Upstream 5′-AAGTCACGCTGGAGTGGTTC-3′
Downstream 5′-CCCCGTCATCAGTTCATACAA-3′
GAPDH
Upstream 5′-TCCGGGTGATGCTTTTCCTAG-3′
Downstream 5′-TTTGCGGTGGAAATGTCCTTTTC-3′

MiR-7, microRNA-7; FAK, focal adhesion kinase; ERK, extracellular signal-regulated kinase; MAPK, mitogen-activated protein kinase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PCR, polymerase chain reaction;