Figure 5. In vivo, FAM83D knockdown inhibited the tumorigenicity, and FAM83D-siRNA transfected SMMC-7721 cells presented an apparent decrease in tumor size and weight comparing the control cells.

A. Expectedly, FAM83D knockdown also reduced the CD44 expression in subcutaneously formed tumor tissues B. In NC group, at least one metastasis with high CD44 expression in lung was observed, but there was no pulmonary metastasis in the FAM83D knockdown group C. In the CTC model, the NC group presented more tumor colonies in lung than the FAM83D knockdown group D. Furthermore, in vitro, the transwell assay confirmed that knockdown of FAM83D would impact migration and invasion of SMCC7721 cells E.