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. 2016 Oct 18;7(47):77508–77515. doi: 10.18632/oncotarget.12716

Figure 4. APTX is a target of miR-424 in cervical cancer.

Figure 4

A. Sequence alignment of miR-424 with the 3′UTR of the APTX gene. B. Hela cells were transfected with indicated nucleotides. After 48 hrs of transfection, APTX expression was measured by Western blot. C. APTX expression was measured by RT-qPCR and Western blot in xenograft tumors from miR-424-overexpressing Hela-XR cells and vector control Hela cells. D. Hela cells were cotransfected with APTX 3′UTR luciferase reporter construct and the indicated nucleotides. After 48 hrs of transfection, the luciferase intensity was assessed. The data are presented as the mean±SD from three independent experiments. ** p<0.01. E. The relative expression of APTX and miR-424 was measured in specimens of cervical cancer patients by RT-qPCR (n=10).