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. 2016 Oct 18;7(47):77543–77557. doi: 10.18632/oncotarget.12721

Figure 4. THC-CBD combination induces autophagic-cell death in MM cell lines.

Figure 4

A. U266 and RPMI were treated with CBD (12.5 μM), THC (12.5 μM) alone and in combination. Lysates of treated cells were separated on SDS-PAGE and probed with anti-LC3, anti-p62 and anti-GAPDH Abs. Blots are representative of one of three separate experiments. Bars represent the densitometric analysis. *p<0.01 vs vehicle and THC treated cells; #p<0.01 vs CBD treated cells. B. U266 and RPMI cell lines were pretreated with BAF1 (50 nM) for 1 h and then treated with CBD (12.5 μM), THC (12.5 μM) alone and in combination for 72 h. Data shown are expressed as mean ± SD of three separate experiments. *p<0.05 vs vehicle treated cells; #p<0.05 vs THC, CBD and BAF1, alone or incombination; §p<0.05 vs THC-CBD; °p<0.05 vs THC, CBD and THC-CBD.