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. 2016 Oct 20;7(47):77707–77720. doi: 10.18632/oncotarget.12774

Figure 2. SIRT1 stimulates Wnt/β-catenin signaling in C3H10T1/2 cells.

Figure 2

A. The mRNA levels of SIRT1 were measured 48h after transfection. Bars indicate SD. **P < 0.01. n = 3 per group. B. The mRNA levels of Wnt signalling pathway of target gene Cyclin D1 was measured by Real-time PCR at 48h after transfection. Bars indicate SD. *P < 0.05. n = 3 per group. C. The protein levels of SIRT1, Cyclin D1 and β-catenin were measured by immunoblotting at 48h after transfection. D. TOPflash/FOPflash was co-transfecred with siRNA of SIRT1 in C3H10T1/2 cells. The luciferase reporter activity was measured at 24h after transfection. Bars indicate SD. **P < 0.01. n = 3 per group. E. C3H10T1/2 cells were co-transfecred with TOPflash/FOPflash, control or siRNA of SIRT1 and with pCMV-HA (control), or pCMV-catenin or pCMV-catenin mutation vector. The luciferase reporter activity was measured at 24h after transfection, and β-catenin were measured by immunoblotting. Bars indicate SD. *P < 0.05; **P < 0.01. n = 3 per group.