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. 2016 Oct 20;7(47):77707–77720. doi: 10.18632/oncotarget.12774

Figure 3. SIRT1 suppresses Wnt signaling antagonists.

Figure 3

A. C3H10T1/2 Cells were treated with 25 mmol/L of resveratrol for 12h, the relatively gene of Wnt signalling were meansured by Real-time PCR Array. Lines below of the center regression lines indicate 2-fold changes in gene expression. n = 3 per group. B. C3H10T1/2 Cells were treated with 10mmol/L nicotinamide for 12h, the relatively gene of wnt signaling were meansured by Real-time PCR Array. Lines above of the center regression lines indicate 2-fold changes in gene expression. n = 3 per group. C. The SIRT1 activity was determined using a SIRT1 fluorometric assay kit at 12h after treatment with resveratrol or nicotinamide. *P < 0.05; **P < 0.01. n = 3 per group. D. Wnt signalling target genes (Cyclin D1 and c-Myc) mRNA levels were meansured by Real-time PCR at 12h after treatment with resveratrol or nicotinamide. *P < 0.05. n = 3 per group. (E-F) C3H10T1/2 cells were infected with shRNA specific for SIRT1 (SIRT1 RNAi), or a scrambled hairpin (Control). E. The protein levels of SIRT1 were measured by immunoblotting. F. Wnt signalling antagonists (sFRPs and Dacts) mRNA levels were meansured by Real-time PCR. n = 3 per group.