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. 2016 Oct 21;7(47):77721–77731. doi: 10.18632/oncotarget.12790

Figure 3. Th1 and Th2 cytokine levels in WT and PD-L2 KO infected mice.

Figure 3

Spleen mononuclear cells were obtained from WT and PD-L2 KO mice orally infected with 8 F. hepatica metacercariae. Then, the cells were stimulated with 80 μg/ml of TE or left unstimulated, and the cytokines measured in culture supernatants by ELISA. A. IFN-γ production by splenocytes at days 3 and 7 p.i. Dots represent mean cytokine levels of individual animals, lines represent the mean value *p<0.05 B. IL-10, IL-4 and IFN-γ production from splenocytes at day 21 p.i. Bars show fold increase of TE stimulated vs unstimulated cells. Data are expressed as mean ± SD *p<0.05;**p<0.005. C. CD4+ and CD8+ splenocytes producing IFN-γ are shown, each dot represents an individual animal *p<0.05. D. CD4+ intrahepatic lymphocytes producing IFN-γ are shown, with each dot representing an individual mouse * p<0.05. One representative experiment is depicted out of two.