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. 2016 Oct 21;7(47):77764–77776. doi: 10.18632/oncotarget.12794

Figure 2. miR-3196 is involved in apoptosis in lung cancer cells.

Figure 2

A. Lung cancer A549 cells transfected with miR-3196 mimic (miR-3196) or negative control miRNA (miRNA.NC) for 24 h were induced with VP16 (100 μM) for 48 h and apoptotic cells were detected by flow cytometry (FCM). The histogram shows the percentage of apoptotic cells including early and late apoptotic cells. B. Lung cancer H1650 cells were transfected with miR-3196 mimic (miR-3196) for 24 h, followed by VP16 (100 μM) treatment for 48 h. The apoptotic cells were detected as in (A). C. A549 cells transfected with miR-3196 inhibitor or its negative control (miR-3196 inhibitor.NC) for 24 h were treated with VP16 (100 μM) for 48 h and the apoptotic cells were subjected to FCM analysis as in (A). D. H1650 cells were induced by VP16 (100 μM) after miR-3196 inhibitor or miRNA inhibitor-NC transfection for 24 h and the apoptotic cells were subjected to FCM analysis as in (A). E. Relative expression of miR-3196 was tested in A549 and H1650 cells after transfected with miR-3196 mimic (miR-3196) or miR-3196 inhibitor. U6 was used as the internal control. Error bars denote the mean ± SD. **P < 0.01, ***P < 0.001 by Student's t test.