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. 2016 Oct 25;7(47):77998–78008. doi: 10.18632/oncotarget.12868

Figure 5. Recombinant STAT3 ELISA reveals STAT3 inhibitors that act at the DNA-binding domain.

Figure 5

Recombinant STAT3 incubated for 1h with A. stattic, B. A18, A26, niclosamide, or C. S3I-1757 prior to addition to ELISA plate, and D. schematic of modified TransAM STAT3 ELISA using recombinant STAT3. Left: Control well demonstrating binding of recombinant STAT3 (blue) to corresponding DNA consensus sequence to produce maximum OD450 (dark yellow). Right: Representative experimental well demonstrating interruption of STAT3 (blue) binding to DNA in the presence of a DBD inhibitor (red) resulting in reduced OD450 (light yellow).