Figure 4. Upregulation of miR-129-3p reverses EMT in HCC cells (high initial metastatic potential).
A. Western blotting and B. immunofluorescence staining assays indicated that the increased expression of epithelial markers (E-cadherin and β-catenin) and the decreased expression of mesenchymal markers (N-cadherin and Vimentin) could be obviously observed in miR-129-3p/mimics-transfected HCCLM3 or MHCC97-H cells, compared with miR-NC/mimics-transfected cells. Each experiment was performed in triplicate.*P<0.05; **P<0.01.