Figure 3.

EE-mediated down-regulation of DAT in the striatum. (a) Time activity curves in the specific brain regions for EE and control groups. While radioactivities in the cerebellum were similar each other, striatal uptake in EE mice was lower than that in control group. (b) Representative brain PET image in EE indicated decreased uptake of the DAT in the striatal region. (c) Binding values of striatal DAT were significantly decreased approximately 18% in the EE mice relative to the control mice at eight weeks post-treatment. (d) The effect of a DAT inhibitor, JHW007. Rotarod tests were performed with accelerating (4–80 rpm) paradigms eight weeks after exposure on either mice from the JHW007-treated EE and control groups or the saline-treated EE and control groups (n = 5 per group). In the pretreatment evaluation, no statistical differences were seen between the groups. At eight weeks post-treatment, the saline-treated EE and JHW007-treated mice induced improvement of rotarod performance relative to the saline-treated control mice.