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. 2017 Feb 10;7:1–10. doi: 10.1016/j.omtn.2017.02.003

Figure 2.

Figure 2

Complex Formation Assessed with PicoGreen Intercalation Assay

(A) Complexes were formed in MQ, incubated at RT for 1 hr, and PicoGreen was added. After 15 min of incubation, fluorescence was measured. The results are normalized to the free siRNA in the same concentration as used in the complexes and RNA free sample. The results are shown as a mean of six separate experiments with error. (B) The formation of PF6/siRNA complexes in HEPES buffer (pH 7.4) or acetate buffer (pH 5.26) is compared to complex formation in MQ.