Figure 4.

FACS Analysis of HDR and NHEJ Events Using the Circular Plasmid RS55

(A) The TLR3 plasmid was cotransfected with nucleases and donor template for the quantification of both HDR and NHEJ events. Artificial NHEJ (TLR3-delTA) and HDR (TLR3-correctGFP) control for the TLR systems were generated through deletion of 2 bp nucleotides (thymin and adenine) and correction of mutant sequence, respectively. (B) FACS data of the respective samples and controls. Statistical analysis: paired t test, NHEJ values compared with T0 plasmid transfection, HDR values compared with RS55 plasmid alone. *p < 0.001; **p < 0.0001; ***p < 0.00005.