Figure 5.

Optimizing HDR Events Using Different Donor Templates

Cas9-mRFP targets and donor templates were coexpressed in the HEK-TLR3 cell line. (A–F) Seventy-two hours after transfection, flow cytometric analysis of mRFP⁺ gated cells displayed GFP⁺ (A, C, and E, HDR repair) and BFP⁺ (B, D, and F, NHEJ repair) cells. The graphs represent triplicate data from three independent experiments. lin., linearized; Pla., plasmid; RS, repair substrate; T0, px459-mRFP without guide RNA. Statistical analysis: ANOVA, *p < 0.05; **p < 0.005; ***p < 0.001.