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Periodic release aliquots confirm release low background release of AD-DOX. Replotting the cumulative release data by the individual release aliquots on a semi-log plot, it is apparent that release from the high affinity AD-DOX/pCD system is two orders of magnitude lower than either release after pH-dependent cleavage and the resulting DOX/pCD system at low pH, or release from either non-affinity pDextran control
