Figure 3.

C-Fos expression (% from baseline control of each experiment) as a function of conditioning and testing treatments in the hippocampus, amygdala, and paraventricular nucleus of the hypothalamus of Sprague Dawley rats, in Experiments 1 to 3. The baseline control of Experiments 1 and 2 was an unmanipulated, home cage control (HCC) group. The baseline control of Experiment 3 was the unpaired-vehicle group corresponding to each mode of administration (i.e. intragastric [i.p.] intubation or intraperitoneal injection [i.p.]). The asterisk in panel C indicates significantly greater C-Fos expression in the PVN, in the ethanol-paired group compared to any of the remaining paired [i.g.] or unpaired groups. The pound sign indicates a significant difference in this structure between the ethanol-paired group and the unmanipulated HCC group. The asterisk and the pound sign in panel D indicate that animals given ethanol at test exhibited significantly less C-Fos in the HPC than those given vehicle at test or than HCC controls, respectively. The asterisk in panel G indicates that animals given chronic ethanol injections exhibited significantly less C-Fos levels in the HPC than those given vehicle injections. The asterisk in panel I indicates that C-Fos activity in the PVN was greater after i.p. than after i.g. administration. Results are expressed as mean ± SEM