Figure 1.
Comparison of Potency and Time Course of Cytotoxicity of Different Adenoviruses in A549 Cells
(A) A549 cells were exposed to EnAd, Ad5, Ad3, Ad11p, and Onyx-015 at different ppc, and viability was measured by MTS assay over 5 days. The dashed line shows 50% cell survival, indicating IC50 values. (B–D) A549 cell growth and cytotoxicity curve under titrated doses of different adenoviruses, measured by xCELLigence cell monitor (Roche). The color of the lines refers to the virus dose shown (see key). (E) A549 cell growth and cytotoxicity curve under titrated doses of cisplatin (500, 100, 20, 4.0, 0.8, and 0 μM, respectively, using the same rank order color code as B–D). (F–H) Cell growth and cytotoxicity graphs for SKOV-3 ovarian carcinoma cells, using the same virus concentrations and color codes as (B)–(D), but with an additional high dose (dark green lines) of 2,500 ppc. In all cases, the vertical arrow shows when the treatment was added. (I–L) Light microscopy images of A549 cells exposed to different cytotoxic treatments; cisplatin (100 μM), Ad5, Ad11p, and EnAd (all 100 ppc), and photographed after 48 hr. Scale bar, 100 μm.