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Detection of ACPP-Cy5 uptake with activated microglia after MCA occlusion. ACPP-Cy5 in 2 nmol contents was i.v. injected 10 min after ischemia and the mice were sacrificed 24 h later. Representative photomicrographs show that immunoreactivity of microglial marker Iba-1, but not astrocytic marker GFAP, correlates with uptake of ACPP-Cy5 in ischemic cortex. Scale bar, 30 µm (right) and 15 µm (left, inset).
