Figure 2.

Neuronal Hif1a or Hif2a deficiency does not affect global brain tissue injury following mild or severe ischemic stroke. Mice were subjected to transient focal ischemic stroke: (a) 60 min of MCAO followed by 24 h reperfusion (n = 10–11, per group) or (b) 30 min of MCAO followed by 72 h reperfusion (n = 7–10, per group). Brains were removed and from each brain, 24 coronal cryosections (10 µm thickness; 0.4 mm distance) were prepared and submitted to cresyl violet staining for quantification of infarct and edema size. Unpaired two-tailed t-test was applied to determine statistical significance.