Fig. 5.

Effect of MAPK inhibition of BCI-215 toxicity. MDA-MB-231 cells were pretreated with concentration gradients of MAPK inhibitors followed by vehicle or a proapoptotic concentration of BCI-215 (25 µM). After 24 hours, cells were stained with Hoechst 33342 and an antibody against cleaved caspase-3, and analyzed for (A) cell density, (B and C) nuclear morphology, and (D) caspase cleavage. Data on graphs depict percent rescue from BCI, calculated as 1 − [(data point + DMSO)/(DMSO + BCI-215)] × 100. Images in (E) illustrate cell loss and nuclear morphology with vehicle (DMSO) and BCI-215 alone, or of BCI-215 in the presence of SCH771984 (375 nM), SB203580 (18 µM), SP600125 (18 µM), or JNK-IN8 (1.8 µM). Data are the averages of 4–7 independent experiments ± S.E.M., each performed in quadruplicate. Images are from an ArrayScan VTI using a 20X objective. Scale bar, 30 µm.