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(A–F) Effect of NaV inhibitors on airway-efferent parasympathetic nerves in the mouse. The effect of (A) TTX, (B) compound 801, (C) compound 13, (D) ICA121431, and ICA121431 (10 µM) in combination with (E) compound 801 and (F) compound 13 on the EFS-induced bronchial contractions, n = 6. (G) Expression of NaV1.1–1.9 subunits in parasympathetic neurons in the mouse. G1–G5, individual ganglia; +, positive control; −, negative control. The arrows indicate expected product size.
