TABLE 1.
The guinea pig primer sequences for single-cell reverse-transcription polymerase chain reaction
| Target | Primer | Sequence (5′ to 3′) | Product Size (bp) | Genomic Size (bp) | NCBI Reference Sequence |
|---|---|---|---|---|---|
| β-actin | Forward | TCTCTTCCAGCCCTCCTTC | 373 | N/Aa | NT_176389.1 |
| Reverse | GTCCTCAAAGGTGCTGTGCT | ||||
| NaV1.1 | Forward | AAGGGTTTCGCTTCTCCATT | 206 | >1000 | XM_003478553.2 |
| Reverse | GTCCTCAAAGGTGCTGTGCT | ||||
| NaV1.2 | Forward | GAGACTTCAGTGGTGCTGGTG | 265 | N/A | XM_003478556.2 |
| Reverse | AGCAGAGACTGGTGTGGAGAA | ||||
| NaV1.3 | Forward | ACACAACAGAGGAGAGGCAGA | 171 | >1000 | XM_003478666.2 |
| Reverse | CGTCTTGGGGAGAATAGGG | ||||
| NaV1.4 | Forward | GGCTCTCCCTCCACCATC | 159 | N/A | XM_003465929.1 |
| Reverse | CAGCGGTTTCTTGCCATC | ||||
| NaV1.5 | Forward | GTCATTTTCCTGGGCTCCTT | 245 | >1000 | XM_005001107.1 |
| Reverse | TTGCTCCTTCTCTCGTGGTT | ||||
| NaV1.6 | Forward | CCTCCTATGGACGAAAAGACA | 222 | >1000 | XM_005006531.1 |
| Reverse | TGCAGATGGTGATAGCCAAG | ||||
| NaV1.7 | Forward | GAGGAAAAGGGAGATGATGAGA | 158 | >1000 | XM_003478661.2 |
| Reverse | AACAGGGAGCCACGAATG | ||||
| NaV1.8 | Forward | CGGAAAGGTGACAATGGAG | 187 | >1000 | XM_003464141.2 |
| Reverse | AGCAGGGACAGTAGCGAAGA | ||||
| NaV1.9 | Forward | GTTCCAGGTTCCCAAATCAA | 162 | >1000 | XM_003464142.2 |
| Reverse | GAGGCAGAAGAGGGTGAGG |
NCBI, National Center for Biotechnology Information.
a
N/A indicates no match in UCSC (University of California, Santa Cruz) Genome Browser in silico polymerase chain reaction.