TABLE 3.
The mouse primer sequences for single-cell reverse-transcription polymerase chain reaction
| Target | Primer | Sequence (5′ to 3′) | Product Size (bp) | Genomic Size (bp) | NCBI Reference Sequence |
|---|---|---|---|---|---|
| β-actin | Forward | GTGGGAATGGGTCAGAAGG | 302 | 756 | NM_007393.3 |
| Reverse | GAGGCATACAGGGACAGCA | ||||
| NaV1.1 | Forward | AGACAGCATCAGGAGGAAGG | 118 | >1000 | NM_018733.2 |
| Reverse | GGAGAACAGGGAACCACGA | ||||
| NaV1.2 | Forward | TTTTCGGCTCATTCTTCACA | 305 | >1000 | NM_001099298.2 |
| Reverse | CATCTCTTGGCTCTGGTCGT | ||||
| NaV1.3 | Forward | AGACAGAGGGAGCACTTGGA | 200 | >1000 | NM_018732.3 |
| Reverse | CTATTGCGTCTTGGGGAAAA | ||||
| NaV1.4 | Forward | TCATCTTCCTGGGTTCCTTC | 206 | 941 | NM_133199.2 |
| Reverse | ATCTGCCTCCTCTCCACCTT | ||||
| NaV1.5 | Forward | TGGGCTCCTTCTACCTTGTG | 247 | >1000 | NM_021544.4 |
| Reverse | CGTTTCCTCCTCTTGCTCCT | ||||
| NaV1.6 | Forward | AGGCAGCAAAGACAAACTGG | 157 | N/A | NM_001077499.2 |
| Reverse | GCAGCACTTGAACCTCTGG | ||||
| NaV1.7 | Forward | ATGCTCTTCTTTGCGGTTTC | 381 | >1000 | NM_001290674.1 |
| Reverse | CGGCTTCTTCCTGCTCTTTT | ||||
| NaV1.8 | Forward | CAATCCGACCCTTACAACCA | 147 | >1000 | NM_001205321.1 |
| Reverse | AAAGACCCCGTCATCCAAG | ||||
| NaV1.9 | Forward | CAGCTTTGGCTGGTCTTTTC | 228 | >1000 | NM_011887.3 |
| Reverse | TTCTCCTTGGCCTCTGTCTC |
NCBI, National Center for Biotechnology Information.
a
N/A indicates no match in UCSC (University of California, Santa Cruz) Genome Browser in silico polymerase chain reaction.