Fig. 6.

MGCD0103 promotes de novo synthesis of PAI-1. (A) qRT-PCR was performed with RNA isolated from cultured NRVFs pretreated with either DMSO vehicle or MGCD0103 for 4 hours followed by the addition of CHX at 30 μM for 18 hours (n = 4 plates of cells/condition). Data represent mean PAI-1:18S expression ± S.E.M. (*P < 0.05 compared with CHX alone). (B) Immunoblot analysis of PAI-1 expression from ARVFs treated as described above with CHX and MGCD0103. Each lane represents an independent plate of cells. MGCD, MGCD0103; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction.