Table 2.
Absorption and emission properties for each fluorescence dye. Absorption percentages for each dye are given for each illumination wavelength. Percentages are normalized for each dye such that the peak absorption wavelength is 100%. Emission spectrum peak wavelengths and spectral width at half maximum are also provided.
| Dye |
|||
|---|---|---|---|
| SR | QS | Fl | |
| Absorption (%) | |||
| 250 nm | 14.5 | 100.0 | 29.1 |
| 375 nm | 5.3 | 15.1 | 1.6 |
| 406 nm | 1.5 | 0.4 | 1.8 |
| 515 nm |
9.5 |
0.0 |
13.1 |
| Emission (nm) | |||
| Peak | 593 | 449 | 515 |
| FWHM | 34 | 94 | 38 |
Note: SR, sulforhodamine; QS, quinine sulfate; Fl, fluorescein.